Simple and sure methodology for massive hepatectomy in the mouse

Tomohide Hori; Norifumi Ohashi; Feng Chen; Ann-Marie T. Baine; Lindsay B. Gardner; Sura Jermanus; Justin H. Nguyen

Authors Tomohide Hori, Norifumi Ohashi, Feng Chen, Ann-Marie T. Baine, Lindsay B. Gardner, Sura Jermanus, Justin H. Nguyen.

Abstract

Abstract

Background. Reliable models for massive hepatectomy in the mouse are required for experimental liver research.

Methods. We analyzed anatomical findings in 100 mice following massive hepatectomy induced by liver reduction >70%. The impact of various factors in the different models was also analyzed, including learning curves, operative time, survival curves and histopathological findings.

Results. According to anatomical results, models with 75%, 80% and 90% hepatectomy produced massive hepatectomy. Learning curves and operative times were most optimal with the clip technique. Each hepatectomy performed using the clip technique produced a reasonable survival curve, and there were no differences in histopathological findings between the suture and clip techniques.

Conclusion. Massive hepatectomy by the clip technique is simple and can provide reliable and relevant data.